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Human GF(Glycosylated Ferritin) ELISA Kit
ELK8575
Size： Price：
48 T $320.00
96 T $458.00
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OverviewPublications

Product name: Human GF(Glycosylated Ferritin) ELISA Kit
Reactivity: Human
Alternative Names: Glycosylated Ferritin; GF
Assay Type: Competitive Inhibition
Sensitivity: 10.21 nmol/mL
Standard: 2000 nmol/mL
Detection Range: 31.25-2000 nmol/mL
Sample Type: serum, plasma
Assay Length: 2h
Research Area: Diabetes
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (nmol/mL) OD Corrected OD
2000.00 0.227
1000.00 0.387
500.00 0.651
250.00 0.895
125.00 1.112
62.50 1.592
31.25 1.799
0.00 2.213

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GF and the recovery rates were calculated by comparing the measured value to the expected amount of GF in samples.
Matrix Recovery range Average
serum(n=5) 95-105% 100%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 92-105% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-99% 83-101% 89-101% 85-97%
EDTA plasma(n=5) 86-95% 93-103% 87-98% 85-96%
Heparin plasma(n=5) 85-92% 87-101% 89-99% 85-95%