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Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) ELISA Kit
ELK8570
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48 T $265.00
96 T $376.00
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Overview

Product name: Human PIVKA-II(Protein Induced by Vitamin K Absence or Antagonist-II) ELISA Kit
Reactivity: Human
Alternative Names: PIVKA-II;  PIVKA II
Assay Type: Sandwich
Sensitivity: 0.129 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Hematology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIVKA-II. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIVKA-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIVKA-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIVKA-II in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.218 2.135
10.00 1.677 1.594
5.00 1.235 1.152
2.50 0.969 0.886
1.25 0.525 0.442
0.63 0.426 0.343
0.32 0.174 0.091
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PIVKA-II and the recovery rates were calculated by comparing the measured value to the expected amount of PIVKA-II in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIVKA-II and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 83-96% 91-103% 96-105%
EDTA plasma(n=5) 85-94% 87-99% 85-101% 79-90%
Heparin plasma(n=5) 86-95% 93-103% 87-98% 85-96%