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Rat eIF4E(eukaryotic initiation factor 4E) ELISA Kit
ELK8552
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat eIF4E(eukaryotic initiation factor 4E) ELISA Kit
Reactivity: Rat
Alternative Names: CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F
Assay Type: Sandwich
Sensitivity: 26.14 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Breast Cancer
Uniprot ID: P63074
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat eIF4E. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat eIF4E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat eIF4E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat eIF4E in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
6000.00 2.231 2.136
3000.00 1.527 1.432
1500.00 1.163 1.068
750.00 0.864 0.769
375.00 0.468 0.373
187.50 0.381 0.286
93.75 0.226 0.131
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant eIF4E and the recovery rates were calculated by comparing the measured value to the expected amount of eIF4E in samples.
Matrix Recovery range Average
serum(n=5) 88-99% 93%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of eIF4E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 88-102% 85-93% 83-97%
EDTA plasma(n=5) 92-105% 83-92% 89-98% 85-94%
Heparin plasma(n=5) 86-102% 87-96% 89-98% 83-103%