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Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit
ELK8534
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48 T $265.00
96 T $376.00
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Overview

Product name: Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit
Reactivity: Human
Alternative Names: C-RP; PTX1; Pentraxin-Related; Pentraxin 1; hs-CRP
Assay Type: Sandwich
Sensitivity: 0.5 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.368 2.282
50.00 1.563 1.477
25.00 1.123 1.037
12.50 0.726 0.640
6.25 0.596 0.510
3.13 0.387 0.301
1.57 0.232 0.146
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-CRP and the recovery rates were calculated by comparing the measured value to the expected amount of hs-CRP in samples.
Matrix Recovery range Average
serum(n=5) 83-101% 87%
EDTA plasma(n=5) 86-94% 90%
Heparin plasma(n=5) 81-93% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 83-96% 93-102% 87-98%
EDTA plasma(n=5) 92-101% 87-98% 85-92% 79-96%
Heparin plasma(n=5) 86-95% 85-96% 89-100% 85-96%