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Human TC(Total cholesterol) ELISA Kit
ELK8420
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Human TC(Total cholesterol) ELISA Kit
Reactivity: Human
Alternative Names: TC
Assay Type: Competitive Inhibition
Sensitivity: 2.76 µg/mL
Standard: 500 µg/mL
Detection Range: 7.82-500 µg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
500.00 0.197
250.00 0.334
125.00 0.596
62.50 0.856
31.25 1.152
15.63 1.583
7.82 1.799
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TC and the recovery rates were calculated by comparing the measured value to the expected amount of TC in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 81-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 85-97% 86-98% 91-103%
EDTA plasma(n=5) 85-96% 87-101% 89-97% 85-94%
Heparin plasma(n=5) 85-94% 79-92% 87-101% 82-90%