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Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit
ELK8404
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat pro interleukin 1 beta (pro-IL1b) ELISA Kit
Reactivity: Rat
Alternative Names: pro-IL1-B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β
Assay Type: Sandwich
Sensitivity: 16.4 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 1.978 1.874
2000.00 1.572 1.468
1000.00 0.996 0.892
500.00 0.821 0.717
250.00 0.539 0.435
125.00 0.308 0.204
62.50 0.252 0.148
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant pro-IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of pro-IL1b in samples.
Matrix Recovery range Average
serum(n=5) 89-107% 98%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 78-90% 84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pro-IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-94% 79-96% 85-99%
EDTA plasma(n=5) 88-97% 86-92% 81-93% 89-105%
Heparin plasma(n=5) 85-94% 79-96% 92-101% 83-90%