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Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit
ELK8402
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48 T $320.00
96 T $458.00
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OverviewPublications

Product name: Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit
Reactivity: Human
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Assay Type: Competitive Inhibition
Sensitivity: 8 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-iso-PGF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-iso-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-iso-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 0.179
1000.00 0.367
500.00 0.527
250.00 0.857
125.00 1.112
62.50 1.537
31.25 1.793
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-iso-PGF2a and the recovery rates were calculated by comparing the measured value to the expected amount of 8-iso-PGF2a in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 92-106% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-iso-PGF2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 87-96% 86-97% 88-95%
EDTA plasma(n=5) 79-93% 89-97% 85-94% 87-101%
Heparin plasma(n=5) 85-94% 87-101% 82-91% 85-97%