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Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit
ELK8374
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48 T $320.00
96 T $458.00
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OverviewPublications

Product name: Mouse 4-HNE(4-Hydroxynonenal) ELISA Kit
Reactivity: Mouse
Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal
Assay Type: Sandwich
Sensitivity: 1.1 ng/mL
Standard: 150 ng/mL
Detection Range: 2.35-150 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
150.00 2.035 1.946
75.00 1.782 1.693
37.50 1.297 1.208
18.75 0.883 0.794
9.38 0.589 0.500
4.69 0.327 0.238
2.35 0.197 0.108
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 84-98% 91%
Heparin plasma(n=5) 80-98% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 96-105% 86-97% 85-101%
EDTA plasma(n=5) 78-91% 86-92% 89-96% 91-97%
Heparin plasma(n=5) 95-104% 97-105% 82-95% 89-97%