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DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit
ELK8370
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit
Reactivity: General
Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate
Assay Type: Competitive Inhibition
Sensitivity: 4.44 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 0.185
200.00 0.397
100.00 0.611
50.00 0.997
25.00 1.139
12.50 1.578
6.25 1.889
0.00 2.101

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant DHEA-S and the recovery rates were calculated by comparing the measured value to the expected amount of DHEA-S in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 88-103% 95%
Heparin plasma(n=5) 95-107% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHEA-S and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 85-96% 89-102% 92-101%
EDTA plasma(n=5) 93-99% 87-101% 85-95% 85-96%
Heparin plasma(n=5) 87-95% 84-98% 95-101% 88-102%