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Rat 6ketoPGF1α(6-keto-PGF1 alpha) ELISA Kit
ELK8363
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat 6ketoPGF1α(6-keto-PGF1 alpha) ELISA Kit
Reactivity: Rat
Alternative Names: 6-keto-PGF1 alpha; 6ketoPGF1α
Assay Type: Competitive Inhibition
Sensitivity: 1.4 pg/mL
Standard: 500 pg/mL
Detection Range: 7.8-500 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 6ketoPGF1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 6ketoPGF1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 6ketoPGF1α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 0.211
250.00 0.413
125.00 0.596
62.50 0.967
31.25 1.197
15.63 1.522
7.82 1.861
0.00 2.132

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 6ketoPGF1α and the recovery rates were calculated by comparing the measured value to the expected amount of 6ketoPGF1α in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 90-103% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 6ketoPGF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 92-105% 79-90% 87-96%
EDTA plasma(n=5) 87-98% 87-100% 85-94% 83-96%
Heparin plasma(n=5) 85-100% 87-96% 82-90% 92-101%