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Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
ELK8351
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human 15-HETE(15-Hydroxyeicosatetraenoic Acid) ELISA Kit
Reactivity: Human
Alternative Names: 15-HETE; 15-Hydroxyeicosatetraenoic Acid)
Assay Type: Competitive Inhibition
Sensitivity: 0.1 ng/mL
Standard: 8 ng/mL
Detection Range: 0.13-8 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 15-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 15-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 15-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
8.00 0.199
4.00 0.433
2.00 0.596
1.00 0.933
0.50 1.156
0.25 1.511
0.13 1.761
0.00 2.211

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 15-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 15-HETE in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 80-94% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 79-97% 96-105% 95-103%
EDTA plasma(n=5) 92-101% 87-98% 85-92% 79-96%
Heparin plasma(n=5) 85-94% 79-96% 92-101% 83-90%