Concentration (μg/mL) | OD | Corrected OD |
---|---|---|
100.00 | 0.191 | |
50.00 | 0.352 | |
25.00 | 0.611 | |
12.50 | 0.833 | |
6.25 | 1.236 | |
3.13 | 1.439 | |
1.57 | 1.793 | |
0.00 | 2.057 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 78-93% | 85% |
EDTA plasma(n=5) | 82-95% | 88% |
Heparin plasma(n=5) | 89-103% | 95% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 87-90% | 87-98% | 89-97% | 85-94% |
EDTA plasma(n=5) | 87-103% | 97-105% | 79-90% | 87-96% |
Heparin plasma(n=5) | 95-106% | 87-105% | 92-101% | 79-90% |