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Simian BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
ELK8167
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Simian BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity: Simian
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 1.18 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma, urine and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Kidney biomarker;
Uniprot ID: P63064
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.266 2.162
100.00 1.592 1.488
50.00 1.194 1.090
25.00 0.958 0.854
12.50 0.496 0.392
6.25 0.357 0.253
3.13 0.258 0.154
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant BMG/b2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of BMG/b2-MG in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 89-107% 98%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMG/b2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-95% 91-106% 87-100% 95-101%
EDTA plasma(n=5) 80-101% 87-96% 96-105% 95-103%
Heparin plasma(n=5) 85-105% 87-98% 91-102% 86-102%