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5-HTP(5-Hydroxytryptophan) ELISA Kit
ELK8124
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: 5-HTP(5-Hydroxytryptophan) ELISA Kit
Reactivity: General
Alternative Names: Oxitriptan; Cincofarm; Levothym; Levotonine; Oxyfan; Telesol; Tript-OH; Triptum
Assay Type: Competitive Inhibition
Sensitivity: 1.74 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Enzyme & Kinase;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HTP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HTP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 0.217
200.00 0.479
100.00 0.793
50.00 0.967
25.00 1.257
12.50 1.596
6.25 1.871
0.00 2.265

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 5-HTP and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HTP in samples.
Matrix Recovery range Average
serum(n=5) 85-95% 90%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HTP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 83-96% 91-103% 96-105%
EDTA plasma(n=5) 95-102% 98-106% 87-98% 91-102%
Heparin plasma(n=5) 88-97% 93-105% 85-99% 90-99%