Products

DDAVP(1-Desamino 8D Arginine Vasopressin) ELISA Kit
One-click to copy product information
ELK7864
Size： Price：
48 T $265.00
96 T $376.00
Manual MSDS Send email to us

Overview

Product name: DDAVP(1-Desamino 8D Arginine Vasopressin) ELISA Kit
Reactivity: General
Alternative Names: d-DAVP; Stimate; Minirin; Desmopressin
Assay Type: Competitive Inhibition
Sensitivity: 38.4 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DDAVP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DDAVP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DDAVP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
10000.00 0.219
5000.00 0.433
2500.00 0.711
1250.00 0.959
625.00 1.275
312.50 1.565
156.25 1.835
0.00 2.316

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant DDAVP and the recovery rates were calculated by comparing the measured value to the expected amount of DDAVP in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 87-99% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DDAVP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-107% 83-99% 80-95% 87-96%
EDTA plasma(n=5) 85-92% 79-96% 89-100% 85-96%
Heparin plasma(n=5) 91-99% 85-97% 85-94% 87-96%