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Human Anti-ALB(Anti-Albumin Antibody) ELISA Kit
ELK7831
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-ALB(Anti-Albumin Antibody) ELISA Kit
Reactivity: Human
Alternative Names: Serum albumin Ab
Assay Type: Competitive Inhibition
Sensitivity: 1.3 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2.5h
Research Area: Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-ALB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-ALB in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.197
100.00 0.357
50.00 0.596
25.00 0.812
12.50 1.197
6.25 1.583
3.13 1.796
0.00 2.112

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-ALB and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-ALB in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-ALB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-101% 86-97% 88-95%
EDTA plasma(n=5) 85-94% 87-96% 92-101% 82-98%
Heparin plasma(n=5) 85-94% 87-101% 86-97% 88-95%