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5-HT(5-Hydroxytryptamine) ELISA Kit
ELK7830
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48 T $265.00
96 T $376.00
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OverviewPublications

Product name: 5-HT(5-Hydroxytryptamine) ELISA Kit
Reactivity: General
Alternative Names: 5HT; Serotonin; N-Methyl-Gamma
Assay Type: Competitive Inhibition
Sensitivity: 3.97 ng/mL
Standard: 900 ng/mL
Detection Range: 14.07-900 ng/mL
Sample Type: serum, plasma, tissue homogenates
Assay Length: 2h
Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
900.00 0.183
450.00 0.385
225.00 0.575
112.50 0.823
56.25 1.157
28.13 1.592
14.07 1.624
0.00 2.256

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.
Matrix Recovery range Average
serum(n=5) 88-103% 95%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 80-95% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 95-104% 87-98% 92-99%
EDTA plasma(n=5) 89-99% 83-96% 98-104% 88-101%
Heparin plasma(n=5) 87-96% 87-96% 96-105% 95-103%