Products

Mouse cTnT/TNNT2(Troponin T Type 2, Cardiac) Microsample ELISA Kit
One-click to copy product information
ELK6207MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Manual Send email to us

Overview

Product name: Mouse cTnT/TNNT2(Troponin T Type 2, Cardiac) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay Type: Sandwich
Sensitivity: 5.6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Uniprot ID: P50752
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.950 1.855
500.00 1.560 1.465
250.00 1.173 1.078
125.00 0.885 0.790
62.50 0.536 0.441
31.25 0.344 0.249
15.63 0.146 0.051
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.
Matrix Recovery range Average
serum(n=5) 83-108% 96%
EDTA plasma(n=5) 95-112% 104%
Heparin plasma(n=5) 83-101% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-112% 85-118% 83-98% 93-100%
EDTA plasma(n=5) 94-116% 89-116% 91-110% 92-111%
Heparin plasma(n=5) 83-102% 82-97% 88-107% 84-108%

Product Components