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Mouse BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
ELK5100
Size： Price：
48 T $320.00
96 T $458.00
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OverviewPublications

Product name: Mouse BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity: Mouse
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 0.16 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Kidney biomarker;
Uniprot ID: P01887
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
300.00 2.119 2.046
150.00 1.663 1.590
75.00 1.267 1.194
37.50 0.874 0.801
18.75 0.528 0.455
9.38 0.362 0.289
4.69 0.232 0.159
0.00 0.073 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant BMG/b2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of BMG/b2-MG in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 83-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMG/b2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 93-101% 87-98% 89-102%
EDTA plasma(n=5) 85-93% 96-103% 97-105% 89-97%
Heparin plasma(n=5) 95-105% 82-95% 81-97% 93-105%