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Human VEGI(Vascular Endothelial Growth Inhibitor) ELISA Kit
ELK5057
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human VEGI(Vascular Endothelial Growth Inhibitor) ELISA Kit
Reactivity: Human
Alternative Names: TNFSF15; TL1; TL1A; VEGI192A; Tumor Necrosis Factor(ligand)Superfamily,Member 15; TNF Superfamily Ligand TL1A; TNF Ligand-Related Molecule 1
Assay Type: Sandwich
Sensitivity: 27.1 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;
Uniprot ID: O95150
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
4000.00 1.946 1.842
2000.00 1.517 1.413
1000.00 1.185 1.081
500.00 0.895 0.791
250.00 0.587 0.483
125.00 0.361 0.257
62.50 0.236 0.132
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VEGI and the recovery rates were calculated by comparing the measured value to the expected amount of VEGI in samples.
Matrix Recovery range Average
serum(n=5) 87-93% 90%
EDTA plasma(n=5) 80-90% 85%
Heparin plasma(n=5) 84-106% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 86-97% 90-98% 80-93%
EDTA plasma(n=5) 82-94% 85-96% 78-90% 91-99%
Heparin plasma(n=5) 82-96% 85-98% 95-104% 82-96%