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Human NT-ProCNP(N-Terminal Pro-C-Type Natriuretic Peptide) ELISA Kit
ELK4727
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48 T $320.00
96 T $458.00
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Overview

Product name: Human NT-ProCNP(N-Terminal Pro-C-Type Natriuretic Peptide) ELISA Kit
Reactivity: Human
Alternative Names: N-CNP
Assay Type: Competitive Inhibition
Sensitivity: 5.49 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Cardiovascular biology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT-ProCNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProCNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProCNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.199
500.00 0.385
250.00 0.567
125.00 0.895
62.50 1.168
31.25 1.537
15.63 1.624
0.00 2.028

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProCNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProCNP in samples.
Matrix Recovery range Average
serum(n=5) 85-98% 91%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 95-107% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProCNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-99% 89-100% 85-96%
EDTA plasma(n=5) 86-95% 93-103% 85-94% 87-101%
Heparin plasma(n=5) 95-102% 90-96% 87-1028% 85-97%