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Human CACYBP(Calcyclin Binding Protein) ELISA Kit
ELK3169
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human CACYBP(Calcyclin Binding Protein) ELISA Kit
Reactivity: Human
Alternative Names: SIP; GIG5; PNAS-107; RP1-102G20.6; S100A6BP; S100A6-binding protein; Siah-interacting protein
Assay Type: Sandwich
Sensitivity: 33 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Developmental science;
Uniprot ID: Q9HB71
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CACYBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CACYBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CACYBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CACYBP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.132 2.050
2500.00 1.571 1.489
1250.00 1.134 1.052
625.00 0.871 0.789
312.50 0.551 0.469
156.25 0.372 0.290
78.13 0.179 0.097
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CACYBP and the recovery rates were calculated by comparing the measured value to the expected amount of CACYBP in samples.
Matrix Recovery range Average
serum(n=5) 86-98% 92%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CACYBP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-94% 87-96% 78-93% 82-90%
EDTA plasma(n=5) 95-102% 89-96% 87-98% 85-93%
Heparin plasma(n=5) 91-103% 86-92% 88-97% 89-97%