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Rat ITaC(Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit
ELK3028
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat ITaC(Interferon Inducible T-Cell Alpha Chemoattractant) ELISA Kit
Reactivity: Rat
Alternative Names: CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine(C-X-C-Motif)ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9
Assay Type: Sandwich
Sensitivity: 19.83 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Signal transduction;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ITaC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 2.218 2.137
1500.00 1.573 1.492
750.00 1.088 1.007
375.00 0.838 0.757
187.50 0.601 0.520
93.75 0.389 0.308
46.88 0.207 0.126
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ITaC and the recovery rates were calculated by comparing the measured value to the expected amount of ITaC in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITaC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 86-92% 87-93% 95-103%
EDTA plasma(n=5) 96-101% 85-92% 79-96% 89-101%
Heparin plasma(n=5) 85-92% 95-103% 97-105% 93-102%