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Rat IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
ELK2603
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
Reactivity: Rat
Alternative Names: FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal
Assay Type: Sandwich
Sensitivity: 0.139 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;
Uniprot ID: P02693
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.989 1.900
10.00 1.628 1.539
5.00 0.976 0.887
2.50 0.782 0.693
1.25 0.457 0.368
0.63 0.357 0.268
0.32 0.236 0.147
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 94-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 96-105% 81-96% 93-107%
EDTA plasma(n=5) 82-96% 85-98% 92-103% 81-95%
Heparin plasma(n=5) 95-102% 83-96% 97-105% 85-97%