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Rat GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit
ELK2494
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48 T $320.00
96 T $458.00
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Overview

Product name: Rat GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit
Reactivity: Rat
Alternative Names: CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim
Assay Type: Sandwich
Sensitivity: 2.8 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GCSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GCSF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.214 2.131
250.00 1.784 1.701
125.00 1.139 1.056
62.50 0.815 0.732
31.25 0.556 0.473
15.63 0.386 0.303
7.82 0.257 0.174
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GCSF and the recovery rates were calculated by comparing the measured value to the expected amount of GCSF in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 78-90% 84%
Heparin plasma(n=5) 84-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-96% 85-99% 79-93% 89-97%
EDTA plasma(n=5) 85-97% 87-96% 85-96% 85-95%
Heparin plasma(n=5) 86-100% 93-108% 80-101% 87-96%