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Human IL-12p40(Interleukin 12 p40) ELISA Kit
ELK2263
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48 T $320.00
96 T $458.00
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Overview

Product name: Human IL-12p40(Interleukin 12 p40) ELISA Kit
Reactivity: Human
Alternative Names: IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit; Interleukin 12B
Assay Type: Sandwich
Sensitivity: 2.8 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Infection immunity;Immune molecule;
Uniprot ID: P29460
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.121 2.025
250.00 1.694 1.598
125.00 1.276 1.180
62.50 0.892 0.796
31.25 0.532 0.436
15.63 0.312 0.216
7.82 0.175 0.079
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-12p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12p40 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 94-107% 100%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 79-92% 83-95% 86-97%
EDTA plasma(n=5) 95-106% 85-101% 87-102% 89-95%
Heparin plasma(n=5) 88-97% 86-92% 91-99% 79-97%