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Human PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
ELK2096
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
Reactivity: Human
Alternative Names: PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2; PTGS2; Prostaglandin Endoperoxide Synthase 2
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;Infection immunity;Reproductive science;Pulmonology;Gastroenterology;Hormone metabolism;
Uniprot ID: P35354
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.126 2.022
10.00 1.628 1.524
5.00 1.136 1.032
2.50 0.864 0.760
1.25 0.589 0.485
0.63 0.319 0.215
0.32 0.249 0.145
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PTGS2/COX-2 and the recovery rates were calculated by comparing the measured value to the expected amount of PTGS2/COX-2 in samples.
Matrix Recovery range Average
serum(n=5) 78-96% 87%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 78-92% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTGS2/COX-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 84-98% 95-106% 88-97%
EDTA plasma(n=5) 86-93% 91-98% 87-98% 99-105%
Heparin plasma(n=5) 89-99% 83-103% 98-104% 96-101%