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Human OXA(Orexin A) ELISA Kit
ELK1465
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48 T $320.00
96 T $458.00
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Overview

Product name: Human OXA(Orexin A) ELISA Kit
Reactivity: Human
Alternative Names: Hypocretin-1
Assay Type: Competitive Inhibition
Sensitivity: 4.93 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Neuro science;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OXA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OXA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OXA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.199
500.00 0.332
250.00 0.527
125.00 0.842
62.50 1.168
31.25 1.457
15.63 1.697
0.00 2.256

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant OXA and the recovery rates were calculated by comparing the measured value to the expected amount of OXA in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 96-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OXA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-95% 88-102% 85-97% 80-92%
EDTA plasma(n=5) 87-96% 91-101% 87-98% 89-103%
Heparin plasma(n=5) 85-101% 84-98% 85-93% 86-98%