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Human E-Cad(E-Cadherin) Microsample ELISA Kit
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ELK12001MS
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Human E-Cad(E-Cadherin) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type: Sandwich
Sensitivity: 2.74 ng/mL
Standard: 500 ng/mL
Detection Range: 7.81-500 ng/mL
Sample Type: Serum, plasma, urine, tissue homogenates and other biological
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Uniprot ID: P12830
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
500.00 2.738 2.660
250.00 1.742 1.664
125.00 1.014 0.936
62.50 0.764 0.686
31.25 0.400 0.322
15.63 0.280 0.202
7.82 0.123 0.045
0.00 0.078 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
Matrix Recovery range Average
serum(n=5) 91-102% 97%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 80-89% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-92% 79-85% 80-90% 91-97%
EDTA plasma(n=5) 88-96% 87-93% 88-95% 88-95%
Heparin plasma(n=5) 94-108% 95-103% 83-90% 87-94%

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