Products

Human E-Cad(E-Cadherin) ELISA Kit
One-click to copy product information
ELK12001
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
Manual MSDS Send email to us

Overview

Product name: Human E-Cad(E-Cadherin) ELISA Kit
Reactivity: Human
Alternative Names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type: Sandwich
Sensitivity: 2.73 ng/mL
Standard: 500 ng/mL
Detection Range: 7.81-500 ng/mL
Sample Type: Serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Uniprot ID: P12830
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
500.00 2.817 2.742
250.00 1.812 1.737
125.00 1.011 0.936
62.50 0.691 0.616
31.25 0.425 0.350
15.63 0.268 0.193
7.82 0.153 0.078
0.00 0.075 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
Matrix Recovery range Average
serum(n=5) 95-106% 101%
EDTA plasma(n=5) 79-88% 84%
Heparin plasma(n=5) 92-99% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 96-105% 93-107% 77-91%
EDTA plasma(n=5) 83-95% 96-104% 77-89% 77-89%
Heparin plasma(n=5) 85-93% 77-83% 87-95% 81-95%