Products

Human TYRP1(Tyrosinase-Related Protein 1) ELISA Kit
One-click to copy product information
ELK11968
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Send email to us

Overview

Product name: Human TYRP1(Tyrosinase-Related Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: TYRP1;5,6-dihydroxyindole-2-carboxylic acid oxidase;DHICA oxidase;Catalase B;Glycoprotein 75 ;Tyrosinase-related protein 1 ;TRP1;TRP;TYRP;CAS2;TYRRP
Assay Type: Sandwich
Sensitivity: 27.4 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Pigment biology;Melanoma tumor
Uniprot ID: P17643
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TYRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TYRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TYRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TYRP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 3.097 3.036
2500.00 2.304 2.243
1250.00 1.775 1.714
625.00 1.316 1.255
312.50 0.615 0.554
156.25 0.354 0.293
78.13 0.169 0.108
0.00 0.061 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TYRP1 and the recovery rates were calculated by comparing the measured value to the expected amount of TYRP1 in samples.
Matrix Recovery range Average
serum(n=5) 87-100% 94%
EDTA plasma(n=5) 92-101% 97%
Heparin plasma(n=5) 94-102% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TYRP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 81-89% 86-95% 96-105%
EDTA plasma(n=5) 94-101% 87-99% 77-89% 77-89%
Heparin plasma(n=5) 92-102% 86-98% 87-99% 95-108%