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Simian sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
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ELK11958
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Simian sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Reactivity: Simian
Alternative Names: MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9
Assay Type: Sandwich
Sensitivity: 13.2 ng/mL
Standard: 2000 ng/mL
Detection Range: 31.25-2000 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.725 2.660
1000.00 1.670 1.605
500.00 0.998 0.933
250.00 0.466 0.401
125.00 0.240 0.175
62.50 0.127 0.062
31.25 0.078 0.013
0.00 0.065 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sC5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of sC5b-9 in samples.
Matrix Recovery range Average
serum(n=5) 95-105% 100%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 86-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sC5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-85% 77-88% 83-97% 81-90%
EDTA plasma(n=5) 92-104% 86-99% 86-100% 86-100%
Heparin plasma(n=5) 83-89% 90-97% 81-95% 87-94%