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Rat PIEZO-1(Piezo-Type mMechanosensitive Ion Channel Component 1) ELISA Kit
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ELK11944
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Rat PIEZO-1(Piezo-Type mMechanosensitive Ion Channel Component 1) ELISA Kit
Reactivity: Rat
Alternative Names: PIEZO1; FAM38A;Membrane protein induced by beta-amyloid treatment
Assay Type: Sandwich
Sensitivity: 21.3 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Uniprot ID: Q0KL00
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 3.413 3.349
2500.00 2.364 2.300
1250.00 1.514 1.450
625.00 0.807 0.743
312.50 0.410 0.346
156.25 0.242 0.178
78.13 0.106 0.042
0.00 0.064 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PIEZO-1 and the recovery rates were calculated by comparing the measured value to the expected amount of PIEZO-1 in samples.
Matrix Recovery range Average
serum(n=5) 82-93% 88%
EDTA plasma(n=5) 94-108% 101%
Heparin plasma(n=5) 84-94% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIEZO-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 84-94% 85-95% 78-86% 77-88%
EDTA plasma(n=5) 94-107% 88-96% 94-103% 94-103%
Heparin plasma(n=5) 86-92% 78-86% 94-100% 78-87%