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Mouse sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
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ELK11935
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Mouse sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
Reactivity: Mouse
Alternative Names: CD354; Triggering Receptor Expressed On Monocytes-1; sTREM1
Assay Type: Sandwich
Sensitivity: 6.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology;
Uniprot ID: Q9JKE2
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sTREM-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sTREM-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sTREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sTREM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.693 2.632
500.00 1.921 1.860
250.00 1.091 1.030
125.00 0.654 0.593
62.50 0.330 0.269
31.25 0.165 0.104
15.63 0.112 0.051
0.00 0.061 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sTREM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of sTREM-1 in samples.
Matrix Recovery range Average
serum(n=5) 80-88% 84%
EDTA plasma(n=5) 93-100% 97%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTREM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-89% 88-99% 93-100% 92-104%
EDTA plasma(n=5) 93-103% 86-95% 82-96% 82-96%
Heparin plasma(n=5) 85-99% 77-89% 81-88% 80-92%