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Mouse MuRF1(muscle-specific RING-finger protein 1) ELISA Kit
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ELK11929
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Mouse MuRF1(muscle-specific RING-finger protein 1) ELISA Kit
Reactivity: Mouse
Alternative Names: TRI63;E3 ubiquitin-protein ligase TRIM63;Muscle-specific RING finger protein 1;MuRF1;Muscle RING finger protein 1;RING-type E3 ubiquitin transferase TRIM63 ;Tripartite motif-containing protein 63;Trim63
Assay Type: Sandwich
Sensitivity: 6.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Cell Biology;
Uniprot ID: Q38HM4
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MuRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MuRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MuRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MuRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 3.199 3.128
500.00 2.325 2.254
250.00 1.549 1.478
125.00 0.778 0.707
62.50 0.365 0.294
31.25 0.224 0.153
15.63 0.117 0.046
0.00 0.071 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MuRF1 and the recovery rates were calculated by comparing the measured value to the expected amount of MuRF1 in samples.
Matrix Recovery range Average
serum(n=5) 85-95% 90%
EDTA plasma(n=5) 88-101% 95%
Heparin plasma(n=5) 86-98% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MuRF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 94-108% 82-94% 91-99% 96-105%
EDTA plasma(n=5) 89-99% 78-87% 88-97% 88-97%
Heparin plasma(n=5) 93-105% 80-93% 86-100% 84-98%