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Simian VEGFR1(Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
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ELK11645
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Simian VEGFR1(Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
Reactivity: Simian
Alternative Names: Flt1; Fms-Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-like tyrosine kinase 1; Tyrosine-protein kinase receptor FLT
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian VEGFR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian VEGFR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian VEGFR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian VEGFR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.354 2.260
2500.00 1.527 1.433
1250.00 1.269 1.175
625.00 0.876 0.782
312.50 0.593 0.499
156.25 0.327 0.233
78.13 0.236 0.142
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VEGFR1 and the recovery rates were calculated by comparing the measured value to the expected amount of VEGFR1 in samples.
Matrix Recovery range Average
serum(n=5) 87-95% 91%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGFR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 82-94% 85-96% 78-90%
EDTA plasma(n=5) 79-94% 87-96% 78-93% 82-90%
Heparin plasma(n=5) 86-102% 87-99% 99-105% 95-104%