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Pig sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
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ELK11327
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Pig sTREM-1(soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit
Reactivity: Pig
Alternative Names: CD354; Triggering Receptor Expressed On Monocytes-1; sTREM1
Assay Type: Sandwich
Sensitivity: 13.1 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.3-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology
Uniprot ID: Q6TYI6
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig sTREM-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig sTREM-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig sTREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig sTREM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.132 2.064
1000.00 1.612 1.544
500.00 1.174 1.106
250.00 0.871 0.803
125.00 0.496 0.428
62.50 0.323 0.255
31.25 0.186 0.118
0.00 0.068 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sTREM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of sTREM-1 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 88-106% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTREM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 89-98% 81-95% 98-105%
EDTA plasma(n=5) 85-99% 98-105% 92-101% 89-105%
Heparin plasma(n=5) 88-96% 81-95% 89-97% 95-106%