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Rat AFM(Afamin) ELISA Kit
ELK11169
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat AFM(Afamin) ELISA Kit
Reactivity: Rat
Alternative Names: ALB2; ALBA; ALF; A-ALB; Alpha-Albumin
Assay Type: Competitive Inhibition
Sensitivity: 0.89 µg/mL
Standard: 200 µg/mL
Detection Range: 3.13-200 µg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Tumor immunity;Infection immunity;Hepatology;
Uniprot ID: P36953
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
200.00 0.189
100.00 0.397
50.00 0.583
25.00 0.857
12.50 1.119
6.25 1.565
3.13 1.789
0.00 2.115

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant AFM and the recovery rates were calculated by comparing the measured value to the expected amount of AFM in samples.
Matrix Recovery range Average
serum(n=5) 95-105% 100%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 92-105% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AFM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 83-96% 87-98% 95-102%
EDTA plasma(n=5) 79-97% 96-104% 85-93% 89-97%
Heparin plasma(n=5) 88-102% 91-98% 86-103% 79-91%