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EasyStep Simian T3(Triiodothyronine) ELISA Kit
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ELK105ES
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64 T $412.00
112 T $588.00
112 T*5 $2499.00
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Overview

Product name: EasyStep Simian T3(Triiodothyronine) ELISA Kit
Reactivity: Simian
Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
Assay Type: Competitive Inhibition
Sensitivity: 0.31 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: Endocrinology;Autoimmunity;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Simian T3 antigen, and the Simian T3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Simian T3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Simian T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 0.106
10.00 0.216
5.00 0.383
2.50 0.701
1.25 1.066
0.63 1.411
0.32 1.766
0.00 2.238

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant T3 and the recovery rates were calculated by comparing the measured value to the expected amount of T3 in samples.
Matrix Recovery range Average
serum(n=5) 84-98% 91%
EDTA plasma(n=5) 82-90% 86%
Heparin plasma(n=5) 83-90% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 77-85% 95-108% 92-101%
EDTA plasma(n=5) 86-92% 87-97% 81-90% 81-90%
Heparin plasma(n=5) 89-100% 94-104% 86-98% 92-102%

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