Products

EasyStep Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
One-click to copy product information
ELK104ES
Size： Price：
64 T $320.00
112 T $458.00
112 T*5 $1947.00
Send email to us

Overview

Product name: EasyStep Rat VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Reactivity: Rat
Alternative Names: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
Assay Type: Sandwich
Sensitivity: 0.032 µg/mL
Standard: 10 µg/mL
Detection Range: 0.16-10 µg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin, and the Rat VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat VE-Cadherin. After TMB substrate solution is added, only those wells that contain Rat VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
10.00 2.511 2.436
5.00 1.829 1.754
2.50 1.130 1.055
1.25 0.575 0.500
0.63 0.261 0.186
0.32 0.189 0.114
0.16 0.141 0.066
0.00 0.075 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
Matrix Recovery range Average
serum(n=5) 86-92% 89%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 85-96% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 95-106% 90-102% 77-83%
EDTA plasma(n=5) 87-97% 95-101% 86-93% 86-93%
Heparin plasma(n=5) 92-102% 92-106% 85-96% 82-90%

Product Components