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Mouse CGRP2(Calcitonin Gene Related Peptide 2) ELISA Kit
ELK1038
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48 T $265.00
96 T $376.00
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Overview

Product name: Mouse CGRP2(Calcitonin Gene Related Peptide 2) ELISA Kit
Reactivity: Mouse
Alternative Names: CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
Assay Type: Sandwich
Sensitivity: 4.68 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Endocrinology;Neuro science;Hormone metabolism;Bone metabolism;Dermatology;
Uniprot ID: Q99JA0
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CGRP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CGRP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CGRP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CGRP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.168 2.110
500.00 1.319 1.261
250.00 0.773 0.715
125.00 0.418 0.360
62.50 0.251 0.193
31.25 0.185 0.127
15.63 0.122 0.064
0.00 0.058 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CGRP2 and the recovery rates were calculated by comparing the measured value to the expected amount of CGRP2 in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 84-97% 90%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CGRP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 81-94% 86-98% 92-101%
EDTA plasma(n=5) 85-96% 86-95% 90-102% 81-90%
Heparin plasma(n=5) 87-95% 83-96% 92-102% 82-93%