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EasyStep Human ALD(Aldosterone) ELISA Kit
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ELK097ES
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: EasyStep Human ALD(Aldosterone) ELISA Kit
Reactivity: Human
Alternative Names: ALD
Assay Type: Competitive Inhibition
Sensitivity: 10.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Extracted urine, serum, plasma
Assay Length: 1.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ALD antigen, and the Human ALD standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human ALD to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human ALD in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 0.195
1000.00 0.397
500.00 0.551
250.00 0.833
125.00 1.139
62.50 1.589
31.25 1.824
0.00 2.256

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ALD and the recovery rates were calculated by comparing the measured value to the expected amount of ALD in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 92-107% 99%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 90-99% 82-101% 93-105%
EDTA plasma(n=5) 92-104% 82-96% 95-102% 87-98%
Heparin plasma(n=5) 87-98% 92-104% 82-96% 95-102%

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