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Human IL-40(Interleukin 40) ELISA Kit
ELK0969
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human IL-40(Interleukin 40) ELISA Kit
Reactivity: Human
Alternative Names: IL-40; UNQ464
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;
Uniprot ID: Q6UX52
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.079 1.981
10.00 1.593 1.495
5.00 1.196 1.098
2.50 0.821 0.723
1.25 0.508 0.410
0.63 0.319 0.221
0.32 0.206 0.108
0.00 0.098 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-40 in samples.
Matrix Recovery range Average
serum(n=5) 84-106% 100%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-105% 95-107% 78-95%
EDTA plasma(n=5) 86-95% 90-103% 87-90% 87-94%
Heparin plasma(n=5) 98-105% 95-107% 92-103% 81-99%