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Mouse anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) IgG ELISA Kit
ELK0963
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse anti-SARS-CoV2(S-RBD) (Omicron,B.1.1.529) IgG ELISA Kit
Reactivity: Mouse
Alternative Names: anti-COVID-19;anti-SARS-CoV-2; anti-2019-nCoV
Assay Type: Sandwich
Sensitivity: 0.91 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and biological fluids
Assay Length: 3.5h
Research Area: Infectious Diseases
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S-BRD IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S-BRD IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S-BRD IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S-BRD IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.201 2.112
50.00 1.612 1.523
25.00 1.273 1.184
12.50 0.871 0.782
6.25 0.539 0.450
3.13 0.357 0.268
1.57 0.241 0.152
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant S-BRD IgG and the recovery rates were calculated by comparing the measured value to the expected amount of S-BRD IgG in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 90-107% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S-BRD IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-99% 85-96% 81-95% 95-105%
EDTA plasma(n=5) 79-92% 82-93% 83-97% 82-103%
Heparin plasma(n=5) 89-99% 85-102% 81-96% 87-105%