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EasyStep Human IgG(Immunoglobulin G) ELISA Kit
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ELK095ES
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48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: EasyStep Human IgG(Immunoglobulin G) ELISA Kit
Reactivity: Human
Alternative Names: Immunoglobulin G
Assay Type: Sandwich
Sensitivity: 1.913 ng/mL
Standard: 200 ng/mL
Detection Range: 3.125-200 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG, and the Human IgG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IgG. After TMB substrate solution is added, only those wells that contain Human IgG and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.207 2.126
100.00 1.657 1.576
50.00 1.263 1.182
25.00 0.841 0.760
12.50 0.539 0.458
6.25 0.366 0.285
3.13 0.236 0.155
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IgG and the recovery rates were calculated by comparing the measured value to the expected amount of IgG in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 88-103% 95%
Heparin plasma(n=5) 92-106% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-101% 82-98% 89-97%
EDTA plasma(n=5) 93-102% 81-96% 93-106% 82-95%
Heparin plasma(n=5) 88-103% 86-97% 87-102% 89-101%

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