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EasyStep Human VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
ELK088ES
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64 T $320.00
112 T $458.00
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Overview

Product name: EasyStep Human VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Reactivity: Human
Alternative Names: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
Assay Type: Sandwich
Sensitivity: 32.1 ng/mL
Standard: 10 μg/mL
Detection Range: 0.16-10 μg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin, and the Human VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VE-Cadherin. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (μg/mL) OD Corrected OD
10.00 2.075 1.987
5.00 1.608 1.520
2.50 1.205 1.117
1.25 0.852 0.764
0.63 0.514 0.426
0.32 0.317 0.229
0.16 0.252 0.164
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-105% 95-107% 82-96%
EDTA plasma(n=5) 88-97% 86-92% 94-103% 89-97%
Heparin plasma(n=5) 96-105% 87-96% 90-98% 92-101%

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