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EasyStep Human IL5(Interleukin 5) ELISA Kit
ELK080ES
Size： Price：
64 T $412.00
112 T $588.00
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Overview

Product name: EasyStep Human IL5(Interleukin 5) ELISA Kit
Reactivity: Human
Alternative Names: IL-5; E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor
Assay Type: Sandwich
Sensitivity: 0.15 pg/mL
Standard: 200 pg/mL
Detection Range: 3.13-200 pg/mL
Sample Type: Serum, plasma, cell culture supernatants
Assay Length: 1.5h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL5, and the Human IL5 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL5. After TMB substrate solution is added, only those wells that contain Human IL5 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
200.00 2.108 2.019
100.00 1.527 1.438
50.00 1.185 1.096
25.00 0.629 0.540
12.50 0.473 0.384
6.25 0.364 0.275
3.13 0.227 0.138
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL5 in samples.
Matrix Recovery range Average
serum(n=5) 78-95% 86%
EDTA plasma(n=5) 80-96% 88%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 82-91% 86-93% 81-94%
EDTA plasma(n=5) 78-96% 86-92% 96-105% 89-103%
Heparin plasma(n=5) 85-94% 87-96% 82-90% 92-101%

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