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Human Anti-AChR(Anti-Acetylcholine Receptor Antibody) ELISA Kit
ELK0781
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-AChR(Anti-Acetylcholine Receptor Antibody) ELISA Kit
Reactivity: Human
Alternative Names: anti-ACHR
Assay Type: Sandwich
Sensitivity: 0.68 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-AChR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-AChR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-AChR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-AChR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.126 2.039
50.00 1.576 1.489
25.00 1.118 1.031
12.50 0.841 0.754
6.25 0.553 0.466
3.13 0.324 0.237
1.57 0.319 0.232
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-AChR and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-AChR in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 78-96% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-AChR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 82-96% 95-102% 87-98%
EDTA plasma(n=5) 87-98% 92-104% 82-96% 95-102%
Heparin plasma(n=5) 89-97% 85-94% 79-96% 85-99%