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Human IFIT-1(Interferon-induced protein with tetratricopeptide repeats 1) ELISA Kit
ELK0759
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48 T $320.00
96 T $458.00
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Overview

Product name: Human IFIT-1(Interferon-induced protein with tetratricopeptide repeats 1) ELISA Kit
Reactivity: Human
Alternative Names: Interferon-induced 56 kDa protein (IFI-56K; P56)
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFIT-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFIT-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFIT-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFIT-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.201 2.098
5.00 1.674 1.571
2.50 1.223 1.120
1.25 0.892 0.789
0.63 0.639 0.536
0.32 0.386 0.283
0.16 0.241 0.138
0.00 0.103 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IFIT-1 and the recovery rates were calculated by comparing the measured value to the expected amount of IFIT-1 in samples.
Matrix Recovery range Average
serum(n=5) 82-94% 88%
EDTA plasma(n=5) 77-95% 86%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFIT-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-102% 83-96% 79-93% 88-97%
EDTA plasma(n=5) 78-97% 86-97% 81-103% 88-97%
Heparin plasma(n=5) 85-92% 83-96% 93-102% 87-98%