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Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit
ELK0755
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse 15d-PGJ2 (15-Deoxy-delta 12,14-prostaglandin J2) ELISA Kit
Reactivity: Mouse
Alternative Names: 15-Deoxy-delta 12,14-prostaglandin J2
Assay Type: Competitive Inhibition
Sensitivity: 1.21 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2.5h
Research Area: Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 15d-PGJ2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 15d-PGJ2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.195
100.00 0.413
50.00 0.612
25.00 0.833
12.50 1.156
6.25 1.511
3.13 1.733
0.00 2.028

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 15d-PGJ2 and the recovery rates were calculated by comparing the measured value to the expected amount of 15d-PGJ2 in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15d-PGJ2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 98-106% 87-98% 91-102%
EDTA plasma(n=5) 88-97% 86-97% 85-103% 89-97%
Heparin plasma(n=5) 92-103% 93-105% 88-101% 97-106%